Candida albicans, a member of the Saccharomycetaceae family and the Candida genus, represents a ubiquitous presence in human environments. These yeasts, typically found in oral cavities, gastrointestinal tracts, and vaginal areas, play a vital role in fermenting certain sugars within the body. However, under specific conditions, C. albicans can transform into an opportunistic pathogen, leading to superficial or systemic candidiasis, particularly in immunocompromised individuals, newborns, and the elderly.
Despite their beneficial role, C. albicans poses risks when it contaminates pharmaceutical and cosmetic products rich in carbohydrates and fatty acids. Ophthalmic solutions, ointments, suppositories, and cosmetics like soaps and powders are particularly susceptible. Detecting C. albicans in these products is critical to prevent potential infections and safeguard consumer health.
Prepare the sample in Trypticasein Soy Broth (TSB) or Buffered Peptone Water at a 1:10 concentration and homogenize. Supplement the stock solution with neutralizers and surfactants if needed.
Spread 10 ml of the stock solution or the equivalent of 1 g/1 ml of the product in 100 ml of Sabouraud Dextrose Broth and incubate at 30-35 ºC for 3-5 days.
Perform subcultures in Sabouraud Dextrose Agar plates and incubate at 30-35 ºC for 24-48 hours.
The growth of white colonies in the medium suggests the possible presence of Candida albicans. Confirmation is conducted through identification tests. A product passes the test if these colonies are absent or if confirmatory tests yield negative results.
Understanding the growth and identification of Candida albicans is vital for ensuring product safety and preventing potential health risks associated with fungal contamination. This comprehensive method provides a reliable framework for detecting C. albicans and mitigating the consequences of its presence in consumer products.
Condalab 1224 | Trypticasein Soy Broth (TSB) EP/USP/ISO 500grams (Minimum ordering quantity is 6 units)
Condalab 1401 | Buffered Peptone Water EP/USP 500grams
Condalab 1205 | Sabouraud Dextrose Broth EP/USP 500grams
Condalab 1024 | Sabouraud Dextrose Agar EP/USP/ISO 500grams