Clostridia, a class of bacteria within the Firmicutes phylum, are predominantly gram-positive anaerobic bacilli. While most genera in this class do not form spores, notable exceptions like Clostridium do. These bacteria are ubiquitous in nature, thriving in soil and the intestinal tracts of various animal species, including humans. They're part of the normal microbiota on mucous membranes and skin, but under certain conditions, they can cause infections ranging from abscesses to colitis.
Distribution and Health Implications
Clostridia's presence spans diverse environments, from natural raw materials to medicinal herbs. Infections they cause can affect numerous body regions, including the gastrointestinal tract, leading to conditions like colitis and appendicitis. Moreover, they're implicated in systemic infections like bacteremia and endocarditis.
Methodologies for Detection
The detection and enumeration of Clostridia involve meticulous laboratory procedures. Sample preparation entails homogenizing the product in a suitable medium, followed by spore inactivation through heating. Primary enrichment involves incubation in specialized media like Reinforced Clostridial Medium, while selective isolation is performed on Columbia Agar under anaerobic conditions. The growth of colonies without catalase activity confirms the presence of Clostridia.
Clostridia's significance in both environmental and clinical settings underscores the importance of accurate detection methods. Understanding their behavior and employing precise laboratory techniques are vital for safeguarding public health and ensuring the quality of various products.
Condalab 1224 | Trypticasein Soy Broth (TSB) EP/USP/ISO 500grams (Minimum ordering quantity is 6 units)
Condalab 1401 | Buffered Peptone Water EP/USP 500grams
Condallab 1007 | Reinforced Clostridial Medium EP/USP 500grams
Condalab 1104 - Columbia Agar Base EP/USP/ISO 500grams (minimum order quantity of 4 units)