Identification and isolation of Pseudomonas Aeruginosa

Unveiling the Colorful World of Pseudomonadaceae: Bacterial Artists in Action
January 16, 2024 by
Abdi Nasser

Bacteria, often associated with illness and infection, can also be surprisingly diverse and intriguing. In the vast microbial world, the Pseudomonadaceae family stands out as a group of bacteria with unique characteristics and an artistic flair. In this blog post, we'll explore the fascinating features of Pseudomonadaceae, a family of straight or curved bacilli that are strict aerobes, Gram-negative, and motile, with no spores. What sets them apart, however, is their ability to create a colorful spectacle through the production of pigments such as pyocyanin and fluoresceine.

Aerobic Marvels:​

 Pseudomonadaceae bacteria thrive in aerobic conditions, meaning they require oxygen to survive. Their straight or curved bacilli shape allows them to navigate through various environments, showcasing their adaptability and resilience. As strict aerobes, they have evolved to harness oxygen for their metabolic processes, contributing to their unique biological characteristics.

Gram-Negative Wonders:

 Gram-negative bacteria like those in the Pseudomonadaceae family possess a distinctive cell wall structure that differentiates them from Gram-positive bacteria. This structural dissimilarity has implications for their response to antibiotics and their overall behavior. Understanding their Gram-negative nature is crucial for effective identification and classification.

Motility and the Absence of Spores: 

Pseudomonadaceae bacteria exhibit motility, a characteristic driven by flagella, thread-like appendages that enable movement. Their ability to move allows them to explore and colonize diverse environments, contributing to their adaptability. Additionally, the absence of spores distinguishes them from other bacterial groups, emphasizing their reliance on other mechanisms for survival and propagation.

The Catalase Positive Identity: 

Identification of Pseudomonadaceae bacteria often involves testing for the presence of catalase, an enzyme that breaks down hydrogen peroxide into water and oxygen. The catalase-positive nature of these bacteria becomes a key diagnostic feature, aiding in their accurate and swift identification.

A Colorful Palette: 

Pyocyanin and Fluoresceine Production: Pseudomonadaceae bacteria are renowned for their artistic ability to produce vibrant pigments, notably pyocyanin and fluoresceine. Pyocyanin, a blue-green pigment, and fluoresceine, a yellow-green pigment, contribute to the striking hues seen in bacterial cultures. Researchers have developed specialized culture media to enhance the production of these pigments, facilitating both identification and the study of their ecological roles.

Culturing for Color: 

Several culture media have been devised to encourage the vivid expression of pyocyanin and fluoresceine by Pseudomonadaceae bacteria. These media not only aid in their identification but also serve as tools for researchers to explore the conditions influencing pigment production. The colorful patterns created by these bacteria open up avenues for studying their ecology and potential applications in various fields.


The Pseudomonadaceae family of bacteria, with its aerobes, Gram-negative nature, and motility, unveils a captivating world of microbial artistry. Their catalase-positive identity and the production of pigments like pyocyanin and fluoresceine add an extra layer of fascination to their biological repertoire. As we continue to delve into the intricate details of microbial life, the Pseudomonadaceae family stands as a testament to the diversity and beauty found in the smallest of Earth's inhabitants

Condalab 2003 - Dey-Engley Neutralizing Broth

Condalab 1244 - Letheen Broth Modified ISO/BAM 500grams 

Condalab 2110 - Eugon LT 100 Broth 500grams

Condalab 4600 - Gram Stain KIT Pack of 4 x 250m

Condalab 11​​02 | Cetrimide Agar Base EP/USP/ISO 500grams

King A Medium (Pseudomonas P) Agar USP/ISO 500grams  

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